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Image Search Results
Journal: Frontiers in Cellular Neuroscience
Article Title: Neuroprotection of IGF-1 in neonatal hypoxic-ischemic brain injury through downregulation of FoXO3a-PUMA pathway
doi: 10.3389/fncel.2025.1685800
Figure Lengend Snippet: Insulin-like growth factor-1 (IGF-1R) expression on neurons is elevated after hypoxic-ischemic (HI) challenge. (A) Real-time PCR (RT-PCR) analysis of IGF-1R in neonatal brain at 7 days after HI or sham operation ( n = 6/group). (B) Representative FACS analysis (left) and statistical analysis (right) of IGF-1R + neurons in neonatal brain at 7 days after HI or sham operation ( n = 6/group). (C) Representative images (left) and statistical analysis (right) of Neun (green) and IGF-1R (red) in the cerebral cortex of mice at 7 days after HI or sham operation ( n = 6/group). Scale bar, 25 μm. Data are mean ± SEM. Statistical differences between two groups were evaluated by two-tailed Student’s t -test. * P < 0.05; ** P < 0.01.
Article Snippet: To examine the percentages of IGF-1 + or IGF-1R + cells, cells were stained with a primary anti-IGF-1 (SinoBiological) or
Techniques: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test